Phytochemical studies on cyanidin 3-O glucoside•HCl from the ripened fruit pulp of Terminalia catappa

Context :Terminalia catappa (Combretaceae) is a large tropical tree grows mainly in the regions of Asia, Africa, and Australia. The fruits drupe 5-7 cm long 3-5.5 broad, green at first, then yellow, finally red when ripe, containing single seed. Ripened fruit pulp contains anthocyanins. Objectives: To extract isolate active anthocyanin from Terminalia catappa. And characterize isolated cyanidin-3-0-glucoside by spectral analysis.Methods: An easy efficient technique for isolating extracting ripen pulp. Besides total phenolic, flavonoid, anthocyanin, reducing sugar content extract, antioxidant capacity T.catappa was evaluated DPPH, H2O2 methods. Characterization cyanidin-3-o-glucoside done UV, IR analysis. While crystal structure elemental detection EDS further structural confirmation LCMS analysis.Result: Fresh contain 26.17 % compound 83.79% content, phenolic found to be 1.58 fresh as compared with gallic acid standard. 34.48 54.50 flavonoid observed.